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This is the first study to investigate androgen metabolism in humans following TRF. However, because of the design of the study, it is difficult to determine the effect of TRF on muscle mass and strength of the participants. This study was designed to examine the effect of TRF on androgen metabolism, but was not designed to examine the effects of TRF on muscle mass or strength of the participants. While the total testosterone concentration was lower in TRF subjects compared to ND subjects, this decrease was not statistically significant. It is plausible that the TRF groups would have a greater decrease in testosterone had the study been of longer duration or if the TRF was of longer duration. Additionally, the diet was modified to match the amount of kilocalories consumed and macronutrient distribution between groups. Therefore, while the study design was not designed to investigate the effects of TRF on muscle mass or strength, the findings may still have an impact on how TRF may impact athletic performance.
Stimulation of steroidogenesis was examined by incubating synovial cells with radiolabeled DHEA, ASD, or testosterone (20 nM; Perkin Elmer, Groningen, The Netherlands) with and without increasing concentrations of the following test compounds: E2, 5≥-DHT, DHT, and progesterone (Sigma). At the end of incubation, metabolites were extracted from the medium and from cells, and all samples were subjected to TLC analysis and analysed by liquid scintillation counting. All assays were performed in triplicate. A competitive binding assay was used to determine the displacement of radiolabeled steroids from specific receptor sites by unlabeled steroids. In this assay, 0.3 ml of medium containing radiolabeled steroids was incubated in the absence and presence of unlabeled steroids at increasing concentrations. The radioactivity of the supernatant was determined by liquid scintillation counting, and the percentage of binding was calculated. The assay was performed with and without in vitro added androgens or oestrogens.
ABCD premiered across India in Hindi, Tamil and Telugu languages. The film was released in the United States on 2 June 2013. As per the Bollywood trade website Box Office India, with around 1.50 billion viewers, the film grossed 45.11 million in its first week. 827ec27edc