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After completion of the MVC procedures, the participants were transferred to the cycle ergotrainer. The participants remained at rest for 5 minutes before performing a 5-min warm-up at 100 W followed by a 5-min recovery (Figure 1). Thereafter, the participants were instructed to perform a 1000-m TT in the shortest possible time. The gear ratio was standardized at the beginning of each TT (5316), but participants were free to change the gear and pedal frequency as desired immediately after the TT had started. The participants were instructed to remain seated throughout test. Feedback of the distance covered was provided every 200 m. Power output and distance were recorded at a frequency of 1 Hz (Tacx Trainer software 3.0, Wassenaar, Netherlands). The O2, respiratory exchange ratio (RER), HR, EMG (5-s recordings), and aerobic and anaerobic mechanical power output (Paer and Pan, respectively) were also determined and statistically analyzed every 200-m. The Pan and Paer were calculated from the RER, O2, and the efficiency estimated during the warm up, in accordance with Hettinga et al. [3]. 153554b96e